Introduction

Buffered saline (Dulbecco) with added calcium and magnesium, concentrated 10x, sterile filtered is a hypertonic solution buffered using phosphate buffer. After dilution it is used for maintaining intracellular and extracellular osmotic balance and stabilization of cells during subcultivation and isolation processes (rinsing of cells, dilution). It provides water for cells and inorganic ions necessary for maintaining cellular metabolism. Formulation with added calcium and magnesium is used as a transport medium or for the preparation of reagents.