Introduction

Buffered saline (Dulbecco) with added calcium and magnesium, sterile filtered is an isotonic solution buffered using phosphate buffer. It is used for maintaining intracellular and extracellular osmotic balance and stabilization of cells during subcultivation and isolation processes (rinsing of cells, dilution). It provides water for cells and inorganic ions necessary for maintaining cellular metabolism. Formulation with added calcium and magnesium is used as a transport medium or for the preparation of reagents.