Introduction

One Step Polymer AP is a polyclonal secondary antibody marked with alkaline phosphatase enzyme (AP) and is used for immunohistochemical staining of samples fixed with formalin or glyoxal and embedded in paraffin blocks. The polymerized secondary antibody technology, which is conjugated with alkaline phosphatase, enables bypassing endogenous biotin and its background signal. This product reduces the number of steps in immunohistochemical staining as it replaces two steps of the traditional biotin-streptavidin procedure. One Step Polymer AP is effective with mouse and rabbit primary antibodies.