Alcian Blue dye is used to prove glycosaminoglycan in mucins, for staining amyloids, cysteines and for polychromatic staining of mastocytes according to Alcian-Blue Safranin. It is also used to determine bacterial species and detecting bacterial capsules. Alcian Blue pH 2.5 kit enables adequate staining and visualization of acid mucins without staining sulfated mucins. Rinsing solutions are of the same pH value, making the specificity of the reaction more intense. Cellular nuclei are stained red using a counterstain.