Bio-Diff 1 reagent is a component of BioGnost’s Bio-Diff kit. It contains methyl alcohol whose primary role istofixsmearas the first step in stainingusing Bio-Diff kit. Other reagents found in the kit is Bio-Diff 2 reagent (red component), Bio-Diff 3 reagent (blue component), and pH 6.8 and 7.2 buffer tablets. Bio-Diff 2 contains eosin Y dye, while Bio-Diff 3 contains azure dyes. Red and blue components’ interaction creates a well-knownRomanowsky effect that cannot be achieved ifeach component is being used individually. Purple color indicates the effect’s presence. BioGnost’s Bio-Diff kit efficiently stains hematology and cytology smears in a short period of time and provides precise staining results, such as results of the May-Gruenwald-Giemsa method. Each part of Bio-Diff kit is stabilized separately and prepared according to the highest standards.