Congo Red Puchtler kit

Product Description

Three-reagent kit for staining amyloids, characteristic by its high ionic strength and pH enhancing the specificity of Congo Red dye binding to amyloid clusters. This method developed by Puchtler remains the gold standard for amyloids in tissue sections. Amyloid clusters have the property of double refraction that enables green coloration under polarized light.

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Introduction

Congo Red Puchtler kit is used for staining amyloids (amorphous clusters) in histology sections. Despite their variable protein composition, amyloid deposits are linked by fibril beta-sheets formation. Congo Red dye binds to fibrils and form a regular pattern of dye molecules during the process. The structural regularity of the dye under polarized light demonstrates double refraction of light by displaying green coloration. High pH level and ionic strength of solutions according to Puchtler achieve higher dye specificity to amyloid.

Technical Data

Trade name: CONGO RED PUCHTLER KIT
Chemical name:
Catalogue number: CRP-X**

Available volumes:
for 100 tests
3x100 mL + 1x30 mL

Storing, stability and expiry date:

Transport information:
Transporting/shipment by road (ADR) - Not classified
Transporting/shipment by sea (IMDG) - Not classified
Transporting/shipment by air (ICAO-TI/IATA-DGR) - Not classified

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