Introduction

BioGnost’s Eosin Contrast Plus is a reagent which is commonly used as a contrast dye for hematoxylin in the histological staining method, the hematoxylin and eosin (HE) staining. This method achieves better cellular structure visualization and differentiation. The microscopic samples’ nuclei are stained blue using hematoxylin, then they are stained various shades of pink and red using cytoplasm eosin. Unlike standard aqueous eosin solutions, alcoholic solution of Eosin Y, Phloxine B, and Biebrich Scarlet (modification of Meter’s Eosin) stain sections more intensely pink and red. Eosin Y, Phloxine B, and BIebrich Scarlett are anion dyes that stain erythrocytes bright red, and it also stains basic cellular components, such as cytoplasm, collagen, and muscle fibers.