Gram Crystal Violet, phenol-free reagent

Product Description

Primary stain reagent for Gram-positive bacteria, doesn’t contain phenol. Crystal Violet is also known as Gentian Violet and Methyl Violet 10B.

Available Options:


Gram Crystal Violet, phenol free reagent is a component of BioGram Eco kit used for differential staining between Gram-positive and Gram-negative bacteria. Gram staining is a method of differentiating bacterial species and it is commonly known and used in microbiology. It is also one of the most frequently used diagnostic methods in hospital and clinical laboratories. Gram staining differentiates bacteria into two groups: Gram-positive and Gram-negative. Division is based on the two groups’ bacterial membrane structural differences, i.e. their capability of retaining the dye. BioGnost’s Gram Lugol solution, stabilized, which is an aqueous solution of iodine and potassium iodide in its composition, also contributes to this ability. During the Gram staining process, it allows the dye to enter the bacterial cell and create insoluble complexes of iodine and primary dye. With this action, it enables color retention and later identification of Gram-positive bacteria.

Technical Data

Chemical name:
Catalogue number: GCF-OT-X**

Available volumes:
100 mL
250 mL
500 mL
1 L
2,5 L

Storing, stability and expiry date:

Transport information:
Transporting/shipment by road (ADR) - UN1170, Ethanol solution, 3, III
ADR: Special provision 144 - Aqueous solution containing 24% or less alcohol volume, not subjected to classification.
Transporting/shipment by sea (IMDG) - UN1170, Ethanol solution, 3, III
Transporting/shipment by air (ICAO-TI/IATA-DGR) - UN1170, Ethanol solution, 3, III
IATA: Special provision A58 - Aqueous solution containing 24% or less alcohol volume, not subjected to classification.

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