BioGnost’s Hematoxylin COL is a formulation of hematoxylin used in histopathology for nuclear cell staining. It can be used instead of Hematoxylin M (Mayer’s Hematoxylin) Unlike Hematoxylin M (used only in progressive staining method), Hematoxylin COL is used in both progressive and regressive staining methods. Hematoxylin is extracted from logwood (Haematoxylon campechianum L.). Hematoxylin oxidizes to hematein and binds with metal ions (mordants), hematein turns into irreplaceable nuclear color. Positively charged hematein-mordant complex then binds with negatively charged phosphate ions of the DNA’s nucleus, creating characteristic blue coloration. BioGnost’s modified Hematoxylin COL is oxidized with iodine and ready for instant use.