Histology, cytology and other related scientific disciplines study the microscopic anatomy of tissues and cells. In order to demonstrate a good tissue and cellular structure, the samples need to be stained in a correct manner. Neutral Red powder dye is used in various staining methods in microscopy. It is used for supravital leukocyte staining, for nuclei staining, and for fluorescent staining. It can also be used as a counterstain during Gram staining, as well as a part of Twort’s histological dye for polychromatic staining.