Hard tissue decalcification is necessary for microscopical analysis of the tested sample in standard histological methods. The sample is completely immersed in the decalcifying solution. The length of time needed for demineralization (decalcification) depends on the size and density of the treated sample. A reagent (based on chelated compound of ethylenediaminetetraacetic acid (EDTA) that binds calcium ions during decalcification of sensitive tissues. That makes tissues softer and ready for further processing.
Test samples are sensitive hard tissues such as the crest of the ilium (Latin Crista iliaca) and keratinized tissue such as blood vessels.