P.A.S.M. / Jones kit

Product Description

Six-reagent Periodic Acid Silver Methenamine kit for staining kidney glomerular basement membranes. Kit includes red counterstain which provides visually rich contrast to target structures stained black.

Available Options:

Introduction

P.A.S.M. / Jones kit is used in histology for visualizing argentaffin structures, especially kidney membranes, but also fungi and certain pathogen organisms. Staining procedure starts with periodic acid solution being used to oxidize 1,2-glycols to aldehydes. During incubation in silver-methenamine-borate working solution aldehydes are reduced and at the same time cause reduction of silver ions to metallic silver that manifests as brown to black structures on the section. This is followed by toning the solution with gold chloride solution that additionally improves staining intensity of target structures (fungi, basal membranes and others), and it reduces background staining. Excessive unbound silver-gold bonds is removed by rinsing the section with sodium thiosulfate solution. Finally, the sections are exposed to Nuclear Fast Red (Kernechtrot) counterstain that stains background structures red; that in turn creates clear and visually rich contrast to target structures (colored in brown-black).

Technical Data

Trade name: P.A.S.M. / JONES KIT
Chemical name:
Catalogue number: PASM-X**

Available volumes:
for 100 tests
5x100 mL + 3x250 mL

Storing, stability and expiry date:

Transport information:
Transporting/shipment by road (ADR) - Not classified
Transporting/shipment by sea (IMDG) - Not classified
Transporting/shipment by air (ICAO-TI/IATA-DGR) - Not classified

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