Introduction

Reticulin Contrast kit is used for identification and easier visualization of argentaffin reticular fibers in connective tissue. Reticulin provides structural support. It is found in the liver, spleen and kidneys. Reticulin fibers are clearly defined in the healthy liver; necrotic and cirrhotic liver has discontinuous fibers. The visualization is based on silver depositions on reticulin fibers. The tissue sample must be oxidized with potassium permanganate. Silver is formed from ammonia solution containing silver nitrate and is deposited in the form of brown sediment on reticulin fibers. Formalin acts as a reducing agent and accelerates the procedure. Unbound silver is washed away using sodium thiosulfate. Reticulin Contrast kit also contains a gold chloride solution that stabilizes and tones the section’s image. The kit contains Nuclear Fast Red (Kernechtrot) counterstain.