Histology, cytology and other related scientific disciplines study the microscopic anatomy of tissues and cells. In order to demonstrate a good tissue and cellular structure, the samples need to be stained in a correct manner. Safranine O powder dye is intended for microscopy staining using different methods. It is most commonly used for staining according to Gram as a counterstain. Also used for staining bacterial spores, demonstration of glycosaminoglycans and nuclei in human and animal samples. Safranine O is a red cation dye used for examining plant anatomy.