Sodium thiosulphate, 2% solution

Product Description

The solution is a component of Grocott, Masson Fontana, P.A.S.M. / Jones, Reticulin, Reticulin Contrast and Von Kossa staining kits.

Available Options:

Introduction

Sodium nitrate, 2% solution is a component of special staining kits, such as Grocott kit, stabilized, PASM/ Jones kit, stabilized, as well as Feulgen kit. Grocott kit and PASM/ Jones are used for visualizing argentaffin structures in histology. For diagnostic purposes it is most commonly used for impregnating basal membranes and fungi using silver. 1,2-glycols are oxidized to aldehydes by treating the section with periodic acid solution. During incubation in silver-methenamine-borate working solution aldehydes are reduced to primary alcohols with simultaneous reduction of silver ions to elementary silver (dark brown-black). Section toning follows using gold chloride solution that enhances target structures staining, as well as removes non-specific staining. Excessive unbound silver-gold bonds is removed by rinsing the section with sodium thiosulfate solution. Finally, the sections are exposed to Fast Green F.C.F. dye (Grocott kit, stabilized) or Nuclear Fast Red dye (PASM/ Jones kit, stabilized) that stains background structures green or red creating a clear and visually rich contrast to brown-black stained target structures.

Feulgen kit is used as cytochemical method of semiquantitative determining DNA in histology and cytology samples.

Technical Data

Trade name: SODIUM THIOSULFATE, 2% SOLUTION
Chemical name:
Catalogue number: NT2-OT-X**

Available volumes:
100 mL

Storing, stability and expiry date:

Transport information:
Transporting/shipment by road (ADR) - Not classified
Transporting/shipment by sea (IMDG) - Not classified
Transporting/shipment by air (ICAO-TI/IATA-DGR) - Not classified

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