TB-Stain Fluorescent kit

Product Description

Three-reagent kit for flourescence-microscopic detection of acid-fast bacteria. Contains TB Auramine-Rhodamine reagent, double amount of TB Decolorizer Fluorescent and TB Permanganate reagent as counterstain.

Available Options:

Introduction

Many bacterial cells are easily stained by using simple dyes or Gram stain. However, a few strains of bacteria, such as Mycobacteria and Nocardia cannot be stained using simple dyes (the results may vary significantly if successfully stained). Cell walls of the Mycobacteria strain contain fatty acids which make them hard to stain. In order to stain the walls, a higher concentration of dye or a longer period of heating is required. However, once stained, the dye is ever more difficult to remove from the cells. Those bacteria are called acid fast because they remain their primary color even after being treated with acid alcohol (3% HCl alcohol solution). Fluorescence has been used to detect acid fast bacteria for many years. This method is more sensitive than the Kinyoun method. It takes less time to interpret the results. Auramine-Rhodamine, acid alcohol (0.75% HCl alcohol solution) as a differentiation medium and potassium permanganate as a contrasting dye are used in this method.

Technical Data

Trade name: TB-STAIN FLUORESCENT KIT
Chemical name:
Catalogue number: TBF-K-X**

Available volumes:
4x100 mL
4x250 mL
4x500 mL

Storing, stability and expiry date:

Transport information:
Transporting/shipment by road (ADR) - Not classified
Transporting/shipment by sea (IMDG) - Not classified
Transporting/shipment by air (ICAO-TI/IATA-DGR) - Not classified

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