Introduction

Histology, cytology and other related scientific disciplines study the microscopic anatomy of tissues and cells. In order to demonstrate a good tissue and cellular structure, the samples need to be stained in a correct manner. Azocarmine G is an anionic dye, very similar to Azocarmine B. It is primarily used with the Azan staining method (according to Heidenhain or various modifications). It is also used in histology polychromatic staining methods, and it is used in histochemistry to dim the background fluorescence in fluorescent staining using Schiff’s reagent.