Bio-Diff 2 reagent is a component of BioGnost’s Bio-Diff kit. Contains eosin Y dye in phosphate buffer. Other reagents found in the kit is Bio-Diff 1 reagent (red component), Bio-Diff 3 reagent (blue component), and pH 6.8 and 7.2 buffer tablets. Bio-Diff 1 reagent contains methyl alcohol, while Bio-Diff 3 contains azure dyes. Red and blue components’ interaction creates a well known Romanowsky effect that cannot be achieved if each component is being used individually. Purple color indicates the effect’s presence. BioGnost’s Bio-Diff kit efficiently stains hematology and cytology sections in a short period of time and provides precise staining results, such as results of the May-Gruenwald-Giemsa method. Each part of Bio-Diff kit is stabilized separately and prepared according to the highest standards.