Colloidal Iron kit

Product Description

Six-reagent kit used for visualization of carboxylated and sulphated groups of acid mucopolysaccharides and proteoglycans. This method can be combined with the PAS method; that way glycogen and neutral mucopolysaccharides would get differentially stained characteristically magenta.

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Introduction

The Colloidal Iron kit is used for visualization of carboxylated and sulphated groups of acid mucins and proteoglycans. This method is based on the principle of binding positively charged ferric ions (Fe3+) to negatively charged endings of acid mucopolysaccharides and proteoglycans. Excessive reagents are rinsed while the bound ferric ions get visualized using the Prussian Blue reaction. In this reaction potassium ferrocyanide causes light blue precipitations of iron ferrocyanide to appear. Finally, the sections are exposed to Van Gieson stain that selectively stains different tissue structures and in turn creates clear and visually rich contrast. The method can be combined with the PAS method; that way glycogen and neutral mucopolysaccharides would get differentially stained characteristically magenta.

Technical Data

Trade name: COLLOIDAL IRON KIT
Chemical name:
Catalogue number: KOL-X**

Available volumes:
for 100 tests

Storing, stability and expiry date:

Transport information:
Transporting/shipment by road (ADR) - Not classified
Transporting/shipment by sea (IMDG) - Not classified
Transporting/shipment by air (ICAO-TI/IATA-DGR) - Not classified

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