Introduction

Grimelius kit is used in histology for visualization of argyrophilic structures in histology tissue sections. Certain tissues, such as neuroendocrine tumors can bind to silver ions from silver nitrate solution, but not reduce them to the visible form – elementary silver; this is why silver ion reduction during staining is achieved by exposing the section to the reducing hydroquinone solution and sodium sulfite. Excessive unbound silver ions are removed by rinsing the section with the sodium thiosulfate solution.