Introduction

Histology, cytology and other related scientific disciplines study the microscopic anatomy of tissues and cells. In order to demonstrate a good tissue and cellular structure, the samples need to be stained in a correct manner. Leishman powder dye is intended for microscopy staining using different methods. It is a typical purple dye for cell nuclei because of its molecular interactions between Eosin Y and Azure B-DNA complex. Staining intensity depends on the amount of Azure B and Azure B/Eosin Y ratio. Leishman stain is classified as a Romanowsky stain. It is used for differentiating leukocytes because staining results in different hues ranging from red to blue.