LeukoGnost ACP kit contains reagents for cytochemical diagnosis of leukemia using bone marrow or whole blood smears. The method is based on the ability of the cellular phosphatase to hidrolyze naphthol AS-BI phosphate in acid environment. The reaction releases naphthol AS-BI that binds diazonium salts to give a red-brown precipitate at the reaction site.
The kit is intended for individual testing of horizontally placed slides and it contains reagents for at least 50 tests for detecting acid phosphatase activity in leukocytes. The reagents are applied by dripping until the entire slide is covered (1-2 mL).