Introduction

LeukoGnost PAS kit contains reagents for cytochemical diagnosis of leukemia using bone marrow or whole blood samples. This staining method is based on the oxidation of intracellular glycogen and neutral mucopolysaccharides in the presence of periodic acid and Schiff’s reagent. Periodic acid cleaves molecules containing glycol groups to create aldehydes which are affected by the Schiff’s reagent in a reaction which stains the cytoplasm magenta. The kit is intended for individual testing of horizontally placed slides and it contains reagents for at least 100 tests for detecting periodic acid-Schiff reagent in leukocytes. The reagents are applied by dripping until the entire slide is covered (1-2 mL).