Introduction

Histology, cytology and other related scientific disciplines study the microscopic anatomy of tissues and cells. In order to achieve a good tissue and cellular structure, the samples need to be stained in a correct manner. Methyl Green powder dye is used in various staining methods in microscopy. It is also used with Pyronine Y dye for one stage DNA (green) and RNA (red) staining method. It is also used as a counterstain with other reactions, such as demonstration of enzymatic activity and for mitochondrial staining according to Altmann.