Introduction

Myco-Mount medium for simultaneous staining the fungi blue and preparing permanent section. Main ingredient of Myco-Mount Blue medium is Lacophenol Blue solution that is one of the most widely used reagents in standard procedures of microscopy analysis of fungi. It is used for preparing permanent microscopic sections that have the fungus cytoplasm stained, creating a bright blue background. That enables detecting hyphal cell wall and other fungal structures that are stained dark blue. The solution consists of several components: phenol (fungicide that causes cellular protein precipitation and inactivation of enzyme systems), lactic acid (acts as a clearing medium), Aniline Blue dye (stains hyalins of fungal structures and in turn making them visible) and mixture of resins and glycols soluble in water (enables permanent state of the section). Because of the components’ properties, BioGnost’s Myco-Mount Blue is at the same time a mounting medium and a staining reagent, enabling practical, fast and efficient sample analysis.