Introduction

Silver ammonia solution is a component of Reticulin and Reticulin Contrast kits used for identification and easy visualization of argentaffin reticulin fibers in connective tissues. Reticulin has supporting function in the body, it is found in the liver, spleen and kidneys. Reticulin fibers are clearly defined with healthy liver; necrotic and cirrhotic liver has discontinuous fibers. The test is based on silver depositions on reticulin fibers. The tissue sample must be oxidized with potassium permanganate. Silver is formed from ammonia solution containing silver nitrate and is deposited in the form of brown sediment on reticulin fibers. Formalin acts as reducing agent and accelerates the procedure. Unbound silver is washed away and removed by using sodium thiosulfate.