Introduction

Van Gieson trichrome kit is used for staining collagen, muscle tissue, keratinized epithelium, cytoplasm, glial fibers, and erythrocytes. Fuchsin Acid Van Gieson is a component of the kit and it contains two dyes (acid fuchsin, picric acid) that simultaneously stain different tissue structures. Acid fuchsin stains collagen fibers intensive red while picric acid stains muscle fibers, erythrocytes and glial fibers yellow. Amyloids, hyalin, colloid and mucosa are stained in nuances between red and yellow. Hematoxylin, Weigert A and Ferri reagent make up Weigert hematoxylin that creates stable cellular nuclei coloration.