Introduction

Weigert-Van Gieson kit is used for staining elastin, connective tissue and collagen. Elastic fibers consist of elastin polymers and elastic microfibrils that make up a 3D network in an extracellular matrix inside connective tissue (skin, elastic cartilage, vascular walls, lung tissue and in vocal cords). Unlike standard histology stains, Weigert van Gieson reagent (known as a resorcin-fuchsin dye) displays selective differentiation of tissue samples, even in the early phase of the disease.