Introduction
Supplements obtained by aseptic collection of horse blood aided by mechanical denaturation of fibrins and reoxygenation.
Defibrination is considered the best method of preventing blood clotting and it is important to be performed quickly with minimal hemolysis and destabilization of erythrocytes structure, although some level of hemolysis is always present in such supplements.
The product is used as a supplement to culture media to determine the type of hemolytic reactions caused by certain microorganisms and to enhance the nutritional value of the media.
Technical Data
The product is used as a supplement to culture media. For more details read the manufacturer's instructions for use for a specific dehydrated medium. Hemolytic reaction with different blood types are not identical. Culture medium intended for use with one blood type may result in unsatisfying hemolytic reactions if used with another blood types.
According to EUCAST recommendation (European committee on antimicrobial susceptibility testing) MH enriched with 5 % defibrinated horse blood (BioSap HO) and 20 mg/L β-NAD is used in the antimicrobial susceptibility testing process using the diffusion method for Streptococcus spp., including S.pneumoniae, Haemophilus spp., M.catarrhalis, L.monocytogenes, Campylobacter spp., P. multocida and other microorganisms.
Preparing the sample:
Leave the bottle at room temperature for 5-10 minutes and gently stir the bottle. Sterilely transfer the appropriate volume of the supplement into the medium cooled to 45–50°C (according to the media manufacturer's instructions).
Storing, stability and expiry date:
Keep BioSap supplements in a closed original packaging at temperature of 2°C to 8°C. Do not freeze and avoid exposing to direct sunlight. The products must not be used after the date printed on the label. Date of manufacture is stated in the product's certificate delivered with each new lot.
