Introduction

The LeukoGnost NAP Kit contains reagents for the cytochemical diagnosis of leukemias on peripheral blood or bone marrow smears. This kit enables differentiation of chronic myeloid leukemia (CML) from a leukemoid reaction and other myeloproliferative disorders. The method is based on the ability of alkaline phosphatase in neutrophil leukocytes to catalyze the hydrolysis of naphthol AS-BI phosphate in an alkaline solution. This reaction produces free naphthol AS-BI, which reacts with diazo salts to form an insoluble blue precipitate at the site of the reaction. The kit is designed for individual testing of horizontally positioned slides and contains reagents sufficient for at least 100 tests for the detection of alkaline phosphatase activity in leukocytes. The reagents are applied dropwise until the entire slide is covered (1–2 mL).