Histology, cytology and other related scientific disciplines study the microscopic anatomy of tissues and cells. In order to demonstrate a good tissue and cellular structure, the samples need to be stained in a correct manner. Sudan III powder dye is used in various staining methods in microscopy. Sudan III is a lysochrome (dye soluble in fats) most commonly used for lipid staining according to Lillie-Ashburn. It is also used for staining other hydrophobic substances.